Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane.[2] This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
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Trypan Blue solution
Details
Category:
detachment enzyme
Manufacture:
Sigma Aldrich
| sterility | sterile-filtered |
| form | liquid |
| storage condition | dry at room temperature |
| concentration | 0,40% |
| shipped in | ambient |
| T8154 | Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture |
| BR717810 | BRAND® counting chamber BLAUBRAND® Neubauer improved without clips, bright-line |
Brochure
CoA
SDS
The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at 0.1cu.mm and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area.
The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.