SureSelect Human All Exon - Hybridization-based Next Generation Sequencing (NGS)

The Agilent SureSelect Human All Exon V7 delivers unmatched coverage of targeted regions with minimal sequencing. Designed using the GRCh38/hg38 genome assembly, this exome design targets only coding regions from RefSeq, CCDS, GENCODE &USC Known Genes. All exon sequencing product features a novel bait design algorithm resulting in an end-to-end design size of only 48.2 MB. A novel bait design algorithm results in a sleek design with an end-to-end design size of only 48.2Mb.

The exome sequencing system provides excellent uniformity and allows more samples to be pooled per flow cell, for more cost-effective sequencing. The accurate targeting ensures that even hard-to-capture exons can be accessed. In addition, the system is compatible with all SureSelect Library prep solutions, making it one of the most versatile exome solutions for a wide range of sample types including low input material and FFPE samples, and workflow needs such as enzymatic or mechanical shearing.

Details

Category:
Life Science – Molecular Biology – Next Generation Sequencing

Manufacture:
Agilent

  • With just 5.3 GB of sequencing, the SureSelect Human All Exon V7 provides 20x coverage of >94% of targeted bases.
  • Excellent overall uniformity and percent on-target enable variant calling with high confidence while reducing sequencing costs.
  • Targets protein coding regions from RefSeq, GENCODE, CCDS and UCSC known genes, including hard-to-capture exons that are omitted from other exomes.
  • Targets all pathogenic variants in the genes included in the ACMG guidelines for secondary findings.
  • The raw data generated from human all exon sequencing can be readily analyzed using the Agilent Alissa Clinical Informatics Software, solving the data analysis bottleneck.
Number of Reactions 16 – 96
Target Region 35.7 Mb
Catalog no. Description
5191-4028 SureSelectXT HS V7, 16 rxn
5191-4029 SureSelectXT HS and Low Input V7, 96 rxn
5191-4004 SureSelectXT Human All Exon V7, 16 rxn
5191-4005 SureSelectXT Human All Exon V7, 96 rxn
5191-4007 SureSelectXT2 Human All Exon V7, 16 rxn
5191-4008 SureSelectXT2 Human All Exon V7, 96 rxn
5191-4010 SureSelectXT Human All Exon V7 Plus 1, 16 rxn
5191-4011 SureSelectXT Human All Exon V7 Plus 1, 96 rxn
5191-4013 SureSelectXT2 Human All Exon V7 Plus 1, 16 rxn
5191-4014 SureSelectXT2 Human All Exon V7 Plus 1, 96 rxn
5191-4016 SureSelectXT Human All Exon V7 Plus 2, 16 rxn
5191-4017 SureSelectXT Human All Exon V7 Plus 2, 96 rxn
5191-4019 SureSelectXT2 Human All Exon V7 Plus 2, 16 rxn
5191-4020 SureSelectXT2 Human All Exon V7 Plus 2, 96 rxn