Maurice - A complete imaged Capillary Electrophoresis Systems

Maurice innovates the conventional capillary electrophoresis technology to automate your protein profiling either by size or charge. Need cIEF and CE-SDS data for your mAbs, ADCs, vaccines or virus-like particles? Maurice does it all. Just pop in one of the preassembled cartridges, drop in your sample vials or a 96-well plate, and hit start. You will complete method development in a day; and get high-resolution, reproducible data in minutes.

Details

Category:
Life Instrument – Capillary Electrophoresis

Manufacture:
Protein Simple

Benefit

Easy to do charge characterization for different molecules. For fusion proteins with complicated charge variants or hydrophobic ADCs, ion exchange chromatography often suffers with poor resolution. Maurice/iCE can provide you significantly improved resolution and reproducible peak pattern, in only 10 minutes.
Analyze mixed proteins easily using Maurice’s cIEF Native Fluorescence. Therapeutic co-mixtures present a challenge for analysis when the two proteins are present at significantly different abundance. Maurice can easily differentiate the two proteins using its native fluorescence feature.
No mess no fuss. Maurice employs a smart, ready-to-use cartridge design which automates column conditioning and clean up. This design affords easy switch of separation modes between cIEF and CE-SDS, without concerns about cross-contamination. Waste is collected inside the cartridge which eliminates the hassle of handling toxic waste such as ADCs. The on-board sample mixing feature further enhances the ease-of-use, and reduces operator-dependent variability commonly encountered with other systems.
Maurice is fast. Maurice produces pI and charge heterogeneity data in less than 10 minutes, and size-based CE-SDS data in 35 minutes. The fast separation time affords a streamlined, robust and automated solution for method development in a day. The icing? You can develop platform methods and use them for multiple molecules too.

Specification

Specifications (for cIEF):
Sample Delivery	:	Vacuum
Typical Separation Time	:	6–10 min (molecule-dependent)
Typical Separation Time	:	6–10 min (molecule-dependent)
Detection Capability	:	UV Absorbance at 280 nm
Detection Capability	:	Fluorescence: Ex 280 nm, Em 320–450 nm
Typical Voltage	:	Pre-focusing: 1500 V, focusing: 3000 V
Sample Injections per Cartridge	:	100 guaranteed, 200 maximum
Maximum Sample Injections per Batch	:	100
pI/Size Range	:	2.85–10.45
pI/Sizing CV	:	1%
CV for Peaks >10% Composition	:	≤5% (Intra-batch), ≤6% (Inter-batch)
Relative Migration Time CV	:	N/A
pI/Sizing Resolution	:	0.05 pI units (for wide range 3–10 ampholytes)
Dynamic Range	:	2 logs
Linearity	:	>0.995
Sensitivity (LOD)	:	0.7 µg/mL (Native fluorescence)
	:	3.0 µg/mL (Absorbance)
	:	(Values based on a monoclonal antibody)

Specifications (for CE-SDS):
Sample Delivery	:	Electrokinetic
Typical Separation Time	:	Reduced IgG: 25 min,
Typical Separation Time	:	Non-reduced IgG: 35 min
Detection Capability	:	UV Absorbance at 220 nm
Detection Capability	:	UV Absorbance at 220 nm
Typical Voltage	:	Separation: 5750 V
Sample Injections per Cartridge	:	100 guaranteed, 200 maximum
Maximum Sample Injections per Batch	:	48
pI/Size Range	:	10–270 kDa
pI/Sizing CV	:	≤2%
CV for Peaks >10% Composition	:	N/A
Relative Migration Time CV	:	<1% for reduced IgG
pI/Sizing Resolution	:	≥1.5 for NGHC/HC IgG Standard
Dynamic Range	:	2 logs
Linearity	:	>0.995
Sensitivity (LOD)	:	0.3 µg/mL
	:	(Value based on Internal Standard)

General Specification:
Minimum Sample Volume	:	50 uL
Sample Tray Options	:	96-well plates or 48 vials
Dimensions	:	44cm H x 42cm W x 61cm D
Weight	:	46 kg (100 lb)

Include	:	Computer with Windows operating system
		1 year warranty

Catalog no.

No. Katalog	Deskripsi
090-000	Maurice
090-001	Maurice S. (CE-SDS)
090-002	Maurice C. (cIEF)

Additional Info

For more information, visit: https://www.proteinsimple.com/maurice.html

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