The IDEXX Swine Influenza Virus Ab Test is an enzyme-linked immunoassay (ELISA) designed to be performed in a diagnostic laboratory or equivalent. The Swine Influenza Virus Ab Test detects antibodies to nucleoprotein (NP) of swine influenza virus in swine serum.
As a blocking ELISA, when antibodies are present in the sample the anti-NP conjugate is blocked from binding to the NP antigen on the plate. Color development is inversely proportional to the number of swine influenza antibodies in the test sample (calculated as a sample-to-negative or S/N value). Antibodies can be detected within 7–14 days of initial infection.