Dimethyl sulfoxide

Dimethyl sulfoxide DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Details

Category:
Cryoprotectant

Manufacture:
Sigma Aldrich

grade Hybri-Max
vapor density 2.7 (vs air)
vapor pressure 0.42 mmHg ( 20 °C)
sterility sterile-filtered
product line BioReagent
D2650 Dimethyl sulfoxide
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
C6164 Cell Freezing Medium-DMSO 1×
sterile-filtered, suitable for cell culture
C9249 CryoSOfree™ DMSO-free Cryopreservation Medium

Brochure
CoA
SDS

DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).