CyFlow® Ploidy Analyser

The CyFlow® Ploidy Analyser is a compact flow cytometer for ploidy analysis, high-resolution DNA and genome size analysis for plants, animals and micro-organisms. This modular system is equipped with a UV LED (365 nm) and/or a green laser (532 nm) which, when used in combination with Partec CyStain® reagents, provides high-resolution DNA analysis with DAPI or propidium iodide. The CyFlow® Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4″” colour display.

This method is time- and cost-saving as it provides precise results quickly with an efficient and validated workflow. For higher throughput, it is possible to equip the system with an Autoloading Station that accepts up to two 96-well plates or 120 test tubes as a single load.

Details

Category:
Life Science – Lab Instrument – Flowcytometer
Animal Health – Lab Instrument – Flowcytometer
Industry – Lab Instrument – Flowcytometer

Manufacture:
Sysmex – Germany

Specification

Optics	1 or 2 optical parameters with selected photomultiplier tubes (PMT) Standard set-up and filters for propidium iodide (PI) and/or DAPI/SSC
Laser option	UV LED (365 nm) green laser (532 nm, 30 mW)
Flow System	Quartz flow cuvette for laminar sample transport and hydrodynamic focusing Sample port with biosafety cleaning function
Volume Measure System	True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement Computer-controlled syringe pump speed, adjustable from 0 – 20 μL/s
Electronics and Signal Processing	Selectable linear or 4-decade logarithmic scale 16-bit analogue-to-digital converters, selectable trigger parameter and individual threshold level settings
Computer System	Integrated Microsoft Windows™ PC with Microsoft Office® Integrated, foldable 15″ colour LCD TFT display Ethernet and USB ports DeskJet colour printer Optional external screen (dual screen mode)
Software	Operating system: Microsoft Windows™ Sysmex Partec operating software for real-time data acquisition, display, analysis and reporting Data format: flow cytometry standard (FCS)

Benefit

Screening for haploids, diploids, triploids and polyploids
Ploidy and genome size analysis in less than two minutes
Detection of anisoploids, allopolyploids and aneuploids
No time-consuming microscopic evaluation

Application

Absolute cell and particle counting
Agroscience and aquaculture: Plant biology and algae
Industrial applications: Detection of ploidy level and Plant genome size and DNA analysis

Catalog no.

Catalog No.	Description
SPG CY-S-3039_V3	CyFlow Ploidy Analyser – UV LED + 532nm 30mW

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