| Absorbance |
|
|
| Plate types |
: |
6 – 384 well plates |
| Wavelength selection |
: |
Double monochromators |
| Wavelength range |
: |
200 – 1000 nm |
| Light source |
: |
Xenon flash lamp |
| Read out range |
: |
0 – 6 Abs |
| Linear measurement range |
: |
0 – 4 Abs (96-well plate) at 450 nm, ± 2% |
|
: |
0 – 3 Abs (384-well plate) at 450 nm, ± 2% |
| Accuracy |
: |
0.003 Abs or ± 2%, at 200 – 399 nm (0 – 2 Abs) |
|
: |
0.003 Abs or ± 1%, at 400 – 1000 nm (0 – 3 Abs) |
| Precision |
: |
SD < 0.001 Abs or CV < 0.5%, at 450 nm (0 – 3 Abs) |
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|
|
| Fluorescence Intensity |
|
|
| Plate types |
: |
6 – 1536 well plates |
| Wavelength selection |
: |
Double excitation and emission monochromators |
| Excitation Wavelength range |
: |
200 – 1000 nm |
| Emission Wavelength range |
: |
270 – 840 nm |
| Light source |
: |
Xenon fl ash lamp |
| Sensitivity |
: |
Top reading: < 0.4 fmol fl uorescein/well (black 384-well plate) |
|
: |
Bottom reading: < 4 fmol fl uorescein/well (clear bottom black |
|
|
384 square well plate) |
| Dynamic range |
: |
Top reading > 6 decades |
|
|
Bottom reading > 5.5 decades |
|
|
|
| Time-resolved fluorescence |
|
|
| Plate types |
: |
6 – 1536 well plates |
| Wavelength selection |
: |
Filters (spectral scanning with double excitation and emission |
|
|
monochromators) |
| Excitation Wavelength range |
: |
Fixed to 334 nm (spectral scanning 200 – 840 nm) |
| Emission Wavelength range |
: |
400 – 700 nm (spectral scanning 270 – 840 nm) |
| Light source |
: |
Xenon fl ash lamp |
| Sensitivity |
: |
< 1 amol Eu/well (white low volume 384 well plate) |
| Dynamic range |
: |
> 6 decades |
|
|
|
| Luminescence |
|
|
| Plate types |
: |
6 – 1536 well plates (spectral scanning 6 – 384 well plates) |
| Wavelength selection |
: |
Direct or filters (spectral scanning with double |
|
|
monochromators) |
| Wavelength range |
: |
360 – 670 nm |
| Sensitivity |
: |
< 7 amol ATP/well (white 384-well plate) |
| Dynamic range |
: |
> 7 decades |
|
|
|
| AlphaScreen |
|
|
| Plate types |
: |
6 – 1536 well plates |
| Wavelength selection |
: |
Filters |
| Excitation wavelength range |
: |
Fixed to 680 nm |
| Emission wavelength range |
: |
400 – 660 nm |
| Light source |
: |
LED |
| Sensitivity |
: |
< 100 amol phosphotyrosine/well (white 384 well plate) |
|
|
|
|
Dispensing |
|
|
| Plate types |
: |
6 – 384 well plates |
| No. of dispensers |
: |
None, one or two |
| Syringe size |
: |
1 ml (standard), 5 ml (optional) |
| Dispensing volume |
: |
2 – 5000 μl, in 1 μl increments (1 ml syringe) |
|
: |
5 – 25 000 μl, in 5 μl increments (5 ml syringe) |
| Accuracy |
: |
< 1μl with 50μl (0.4 mm tip), < 0.2 μl with 5μl (0.25 mm tip) |
| Precision |
: |
< 1μl with 50μl (0.4 mm tip), < 0.25μl with 5μl (0.25 mm tip) |
| Dead volume |
: |
Reagent loss < 100 μl, Total tubing volume < 800 μl |
|
|
|
| Incubator and Shaker |
|
|
| Temperature range |
: |
From ambient + 4°C to 45 °C |
| Shaking type |
: |
Orbital |
|
|
|
| General Features |
|
|
| Measurement modes |
: |
End-point, kinetic, spectra, multipoint and kinetic spectra |
| Measurement speed |
: |
Reads a 96-well plate in 15 s, a 384-well plate in 45 s, |
|
|
and a 1536-well plate in 135 s (minimum times) |
| Interface |
: |
PC software (SkanIt software) |
| Dimensions (D x W x H) |
: |
58 x 53 x 51 cm (23 x 21 x 20 in) |
| Weight |
: |
56kg |
| Application |
: |
DNA/RNA quantification
Protein quantification
Reporter gene assays
Cellular assays
Signal transduction
Toxicity assays
Ca2+ flux assays
Kinase assays
Ion channel assays
Hormone assays
Enzyme acitivty assays
Cell proliferation assays
Immunoassays (ELISA, Fluorescence Immunoassays)
Apoptosis assays
FRET, TR-FRET, BRET assays |
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|
