Vitamin and Nutrient Mixture

M&S Basal Medium contains macronutrients that
include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Details

Category:
Vitamin and Nutrient Mixture

Manufacture:
Sigma Aldrich

Specification

form

solid

Catalog no.

A3795	Ammonium nitrate plant cell culture tested
B6768	Boric acid BioReagent, for molecular biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
C7902	Calcium chloride dihydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell c ulture, ≥99.0%
C1416	Chu (N6) Basal Salt Mixture, powder, plant cell culture tested
255599	Cobalt(ii) chloride hexahydrate, 98%
C5915	Coconut water, suitable for plant cell culture
2031	D-(+)-Biotin 1 gr
G1019	Gamborg’s Vitamin Solution 1000 ×, liquid, plant c ell c ulture tested
F8263	Iron(II) sulfate heptahydrate plant cell culture tested, ≥99%
K3129	Kao and Michayluk Vitamin Solution 100 ×, liquid, suitable for plant cell culture
M7150	Murashige and Skoog Vitamin Powder, 1000 ×, powder, plant cell culture tested
M3900	Murashige and Skoog Vitamin Solution 1000 ×, liquid, plant cell culture tested
I7508	myo-Inositol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
N0761	100GNicotinic acid BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%
C7290	N-Z-Amine® A, plant cell culture tested
P5655	Potassium phosphate monobasic powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
T1270	Thiamine hydrochloride BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Resources

Brochure
CoA
SDS

Application note

A sulfur-containing vitamin that plays a role in numerous carboxylation and deamination reactions.

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