Trypsin-EDTA solution

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys – lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys – Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.


detachment enzyme

Sigma Aldrich

sterilitysterile; sterile-filtered
product lineBioReagent
concentration1 ×
application(s)cell culture | mammalian: suitable
impuritiesPorcine parvovirus, none detected (9 CFR)
pH-range7.0 – 7.6
storage temp.−20°C
T3924Trypsin-EDTA solution
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
T3449TrypZean® Solution, 1×
recombinant, expressed in corn, sterile-filtered


The typical use for this product is in removing
adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.