The EnSight® is a high-performance and configurable Multimode Plate Reader optimal for orthogonal approaches. The instrument offers HTS Alpha, Ultra-sensitive Luminescence and fast well-imaging technologies alongside conventional multimode detection technologies. It has been designed for laboratories with diverse user needs for basic research, assay development or medium-throughput screening such as secondary screening or hit confirmation.
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PerkinElmer EnSight Multimode Plate Reader
Details
Category:
Microplate Reader
Manufacture:
PerkinElmer
- Fluorescence well-imaging with single cell resolution for up to four colors (with excitation of three colors in parallel), brightfield and digital phase-contrast imaging
- AlphaLISA® / AlphaScreen® detection with high power laser excitation
- Fluorescence intensity detection (top- and bottom-reading) with monochromator
- Time-resolved fluorescence detection, for example DELFIA® and LANCE® technologies
- Ultra-sensitive luminescence for low signals
- Filter- and optional monochromator-based absorbance detection
- Well-imaging, Alpha technology and conventional multimode detection technologies all on asingle system
- Fast well-imaging with online data analysis in minutes
- Easily configurable and field upgradable
- Base unit with filter-based absorbance technology
- Eight barcode identified filter positions
- Kaleido™ Data Acquisition and Analysis Software
- With integrated data analysis, ready-made protocols and various data export options
| Plate Formats | : | 6- to 384-wells |
| Temperature control
| :
| Temperature range 3 °C above ambient up to 65 °C (0.1 °C increments) Uniformity +/- 1 °C |
| Shaking | : | Linear, orbital and double orbital |
| Monochromator | : | Bandwidth <8 nm (FWHM) |
| Wavelength range (increments): 230-1000 nm (excitation), 0.5 nm increments; 230 – 850 nm (emission), 0.5 nm increments | ||
| Dimensions | : | Height: 56.4 cm (22.2 in.); depth: 63.2 cm (24.9 in.); width: 46.0 cm (18.1 in.); weight: 75* kg (165* lb) *depending configuration |
| Electrical requirements
| :
| Power consumption: 150-300 VA (instrument); 100 VA (monitor); 280 VA (PC). Main voltage: 100-240 V, 50/60 Hz (instrument); 100-240 V, 50/60 Hz (monitor) |
| Operating system | : | Windows® 10 (64 bit) |
| Imaging Available light sources (LED) |
: |
LED 385 nm, 470 nm, 525 nm, 635 nm (brightfield 740 nm) |
| Detector | : | sCMOS-camera |
| Emission wavelength | : | 400-750 nm |
| Objective | : | 4x |
| Resolution | : | 7 μm |
| AlphaScreen (Phosphotyrosine (PT66)) | ||
| 384-well plate | : | < 100 amol |
| Fluorescence (Fluorescein) *96-well plate | ||
| Sensitivity (top-reading) | : | < 1 fmol/well |
| Sensitivity (bottom-reading) | : | Monochromator wavelength range: 230-850 nm (0.5 nm step) Monochromator bandwidth (Exc. and Em.): < 8 nm (FWHM) |
| Time-resolved fluorescence (Europium) *96-well plate | : | Sensitivity: <32 amol/well |
| Absorbance (Filter) *96-well plate | : | Dynamic range 0-4 OD Accuracy @ 2 optical density < 2 % Precision @ 2 optical density < 0.5 % Wavelength range 230-1000 nm |
| Absorbance (Monochromator) *96-well plate | : | Dynamic range 0-4 OD Accuracy @ 2 optical density < 2 % Precision @ 2 optical density < 0.5 % Wavelength range 230-1000 nm (0.5 nm step) Monochromator bandwidth <8 nm (FWHM) |
| Luminometry (ATP) *96-well plate | : | Sensitivity (glow) < 10 pM |
| Catalog no. | Description |
| HH34011000 | EnSight Base Instrument Assembly |