Jess - Your complete Protein Analysis Solution

Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed results in 3 hours. Go further with multiplexing—her fluorescent detection gets all the information you need in one shot. Jess, she’s like Western blot meets ELISA in one.


Life Instrument – Capillary Electrophoresis

Protein Simple

  • Easy to do and fast result.  All assay steps from protein separation, immunoprobing, detection and analysis of data are fully automated in Jess. With just 30 minutes of experimental setup (sample preparation and loading into the plate) and 3 hours of hands-free run time, you can be analyzing data for your next publication or grant.
  • Analyze 13-25 samples in one go. Got a lot of samples? Jess can dramatically increase your throughput. She automates the protein separation and immunodetection of traditional Western blotting, giving you fully analyzed results in just 3 hours. Everything happens inside a capillary, and Jess precisely controls the protein separation and reagent additions and incubations, even the detection. Use Jess’s fluorescent capabilities and multiplex your proteins for even higher throughput. With her 13 and 25 sample capillary cartridges you’ll get the throughput you need, with minimum hands-on time.
  • Get a quantitative Western Blot data using Jess. With Jess, protein quantitation is a breeze. At the conclusion of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of protein size and concentration. With a few clicks you’ll be analyzing immunoassay-like standard curves and precisely quantifying your protein. Multiplexing takes your options to the max, helping you identify and differentiate relative protein expression between samples in one shot. And with in-built protein normalization, you’ll have the confidence you need in your results, experiment after experiment.
  • Multiplexing assay using Jess Fluorescent assay. Jess lets you Superplex, so you can do everything in one run. Like multiplexed chemiluminescent and fluorescent assays on the same sample at the same time. Or look at multiple targets and parameters simultaneously. Or get info on protein abundance, isoform and size all at once. Or add total protein without giving up a detection channel. Or detect high and low abundance proteins in the same sample, even when they have identical molecular weights. Or do chemiluminescent and fluorescent multiplexing assays in parallel just to be absolutely sure you didn’t miss a protein.
  • Easy to do protein normalization using Jess. Total protein detection happens in the same capillary on the same sample, but runs on a separate channel from NIR so you get to keep your existing IR, NIR and chemiluminescence channels. Jess’ same-time detection also gives you more consistent, reliable results than traditional assays without having to stain and wash a single blot.
  • Western Blot using small volume of sample using Jess. Jess gets you down to picogram-level sensitivity with just minimum 3 µL of sample. Tired of stripping and reprobing? Jess’s fluorescent detection lets you maximize the data you get in one shot.
  • Jess can be used as an imager using chemiluminescent blot imaging system. If you’re still doing traditional western blot, Jess can be used as an imager. You can also compare your traditional western blot result with Simple Western result using Jess instrument.
Sample Required:0.3-1.2 ug (Total Protein Specification)
 :0.6-1.2 ug (Chemiluminescence Specification)
 :2-4 ug (Fluorescence Specification)
 :0.6-4 ug (Protein Normalization Specification)
Volume Required:3 µL/well
Size Range:Molecular weight (MW) ladder ranges from 2-440 kDa
Sizing CV:<10%
Intra-assay CV:<15%
Inter-assay CV:<20%
Resolution (± percent difference in MW):± 15-20% for MW <20 kDA
:± 10% for MW >20 kDa
Quantitation CV:<20%
Dynamic Range:2-3 logs, 3-4 logs
Sensitivity:ng, low pg, high pg
Capillary:5 cm, 100 µm, 400 nL
Run time:<3hours
Samples per run:13 or 25
Weight:23 kg
Dimensions (closed):0.36 m H X 0.3 m W X 0.57 m D
Dimensions (open):0.36 m H x 0.53 m W x 0.57 m D
Power:US/CAN 120 V AC, 60 hz, 4.2 amps
:Europe 240 AC, 50 Hz, 2.1 amps
:Japan 100 AC, 50/60 Hz, 5.0 amps
Operating temperature:18-24 °C
Operating humidity:20-60% relative, non-condensing
Include:Computer with Windows operating system
1 Western Blot membrane imaging Catridge
1 year warranty