Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.
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Fetal Bovine Serum
Details
Category:
Serum
Manufacture:
Sigma Aldrich
| Mycoplasma | None detected (Broth Culture) |
| Osmolality | 260 – 340 mOsm/kg H2O |
| Bacteriophage Testing | Record (PFU/mL) |
| Total Protein | 3.0 – 4.5 g% |
| Chemical Analysis | Cholesterol – Report result |
| Hormone Level | Estradiol – Report result |
| Chemical Analysis | Iron – Report result |
| Glucose – Report result | |
| Cloning Assay | Pass |
| Mycoplasma | None detected (DNA Fluorochrome Stain) |
| Insect Cell Culture Test | Min CD 1.2X10E6, Max DT 33.5 hr |
| Cell Lines | Record cell lines used |
| pH | 6.7 – 8.0 |
| Bovine IgG | <= 1 mg/mL |
| Endotoxin | <= 10 EU/mL |
| Serum Performance Test | Pass |
| Cell Lines | Record Cell Lines Used FIO |
| Hemoglobin | <= 20 mg% |
| F2442 | Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma |
| F7524* | Fetal Bovine Serum non-USA origin, sterile-filtered, suitable for cell culture |
Brochure
CoA
SDS
FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture.[4][5][1] It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.